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3 edition of Instability of simple sequence DNA in Saccharomyces cervisiae found in the catalog.

Instability of simple sequence DNA in Saccharomyces cervisiae

Instability of simple sequence DNA in Saccharomyces cervisiae

  • 353 Want to read
  • 35 Currently reading

Published .
Written in English

    Subjects:
  • Eukaryotic cells.,
  • Saccharomyces cerevisiae.,
  • Nucleotide sequence.

  • Edition Notes

    Statementby Samuel T. Henderson.
    Classifications
    LC ClassificationsMicrofilm 94/2542 (Q)
    The Physical Object
    FormatMicroform
    Paginationviii, 109 leaves
    Number of Pages109
    ID Numbers
    Open LibraryOL1241998M
    LC Control Number94628752

    ing the rates at which GCRs occur in Saccharomyces cerevisiae. These assays, and modified versions of them, select for progeny that lose a nonessential chromosome arm due to a GCR mediated by either non-repetitive [7–10], low-copy repeat [9,11], or high-copy repeat DNA [12] and allow quantitative genetic analysis of the pathways.   Abstract. A partially purified primase-polymerase complex from the yeast, Saccharomyces cerevisiae was capable of replicating a single stranded circular phage DNA into a replicative form with high efficiency. The primase-polymerase complex exhibited primase activity and polymerase activity on singly primed circular ssDNA as well as on gapped by: 8.

      Abstract. Using a biochemical approach, we have detected an activity in Saccharomyces cerevisiae extract that displays the same DNA binding specificity as the mammalian E2F transcription factor and interacts with TTTCGCGC promoter elements. Additional studies revealed that this factor, termed SCELA (S. cerevisiae E2F-like activity), also binds to the closely related SCB promoter . Abstract. Yeast 2-μm DNA is a closed-circular extra-chromosomal DNA element of which 50– copies are normally found in several strains of Saccharomyces gene products of this DNA element are known and a direct function has not yet been established. To obtain information on possible gene products of this molecule, we engaged in a study of the expression of cloned yeast 2-μm Author: C. P. Hollenberg, H. D. Royer.

    The genome sequence and gene prediction of Saccharomyces cerevisiae strain SC have not been determined by the JGI, but were downloaded from the Saccharomyces Genome Database (SGD, ) on November 30th Please note that this copy of the genome is not maintained by SGD and is therefore not automatically updated.   In order to better understand the involvement of the DNA molecule in the replication initiation process we have characterized the structure of the DNA at Autonomously Replicating Sequences (ARSs) in Saccharomyces cerevisiae. Using a new method for anti-bent DNA analysis, which allowed us to take into account the bending contribution of each successive base plate, we have Cited by:


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Instability of simple sequence DNA in Saccharomyces cervisiae Download PDF EPUB FB2

Instability of simple sequence DNA in Article (PDF Available) in Molecular and Cellular Biology 12(6) July with 36 Reads How we measure 'reads'.

Instability of simple sequence DNA in Saccharomyces cerevisiae. S T Henderson and T D Petes We examined the instability of poly(GT) and poly(G) tracts in the yeast Saccharomyces cerevisiae. We found that these tracts were dramatically unstable, altering length at Cited by: Henderson ST, Petes TD.

Instability of simple sequence DNA in Saccharomyces cerevisiae. Mol Cell Biol. Jun; 12 (6)– [PMC free article] Hinnebusch AG. Evidence for translational regulation of the activator of general amino acid control in yeast. Proc Natl Acad Sci U S A. Oct; 81 (20)– [PMC free article]Cited by:   Assays for Detecting Genome Instability.

The utility of S. cerevisiae systems for studying genome rearrangements was first recognized nearly 20 years ago. In these initial assays, an extra copy of a DNA sequence was inserted at a site on an unrelated chromosome (ectopic site), and this was followed by selection for recombination between the ectopic sequences ().Cited by: Saccharomyces cerevisiae (/ ˌ s ɛr ə ˈ v ɪ s i.

iː /) is a species of has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes (one can see the yeast as a component of the thin white film on the skins of some dark-colored fruits such as plums; it exists among the waxes of the cuticle).Class: Saccharomycetes.

RNA-DNA sequence differences in Saccharomyces cerevisiae Article (PDF Available) in Genome Research 26(11) September with 59 Reads How we measure 'reads'. Media for propagation of strains and FC media containing both 5-fluoroorotic acid (5-FOA) and Canavanine (Can) for determining GCR rates were as previously S.

cerevisiae strains were propagated at 30 °C. Strains used in this study were all made by standard PCR based gene disruption methods and correct gene disruptions were verified by PCR as previously described.Cited by: The technique of determining the DNA sequence of large genomes has been unchanged for 21 years1.

Sequencing the yeast genome required considerable organization by the European Union, which initi-ated the grouping of 35 laboratories to sequence the first yeast chromo-some2 and coordinate an international effort to sequence the others. The complete nucleotide sequence of Saccharomyces cerevisiae chromosome X( bp) reveals a total ofopenreadingframes(ORFs), the coding region covering %dredand eighteen ORFs(31%) correspond to genes previously identified in siae.

All other ORFs represent novel putative yeastgenes, whosefunctionwill. Molecular Analysis of Saccharomyces cerevisiae Chromosome I On the Number of Genes and the Identification of Essential Genes DNA sequence analysis of these mutant alleles and of the corresponding wild-type region revea,led that each mutation was a single interval by RNA-DNA blot-hybridization analyses (Diehl & Pringle, ).

The large. Mutations in the human RecQ4 DNA helicase are associated with three different diseases characterized by genomic instability. To gain insight into how RecQ4 dysfunction leads to these pathologies, several groups have used the Saccharomyces cerevisiae RecQ4 homolog Hrq1 as an experimental model.

Hrq1 displays many of the same functions as RecQ4 in vivo and in by: 8. Abstract. We determined the complete sequence of 71 bp-long mitochondrial genome from Saccharomyces paradoxus entirely by direct sequencing of purified mitochondrial DNA (mtDNA).

This mtDNA possesses the same features as its close relative Saccharomyces cerevisiae – A + T content %, set of genes coding for the three components of cytochrome oxidase, cytochrome b, Cited by:   Upon exposure to agents that damage DNA, Saccharomyces cerevisiae undergo widespread reprogramming of gene expression.

Such a vast response may be due not only to damage to DNA but also damage to proteins, RNA, and lipids. Here the transcriptional response of S. cerevisiae specifically induced by DNA damage was discerned by exposing S.

cerevisiae to a panel of three Cited by: The yeast Saccharomyces cerevisiaeis the pre-eminent organism for the study of basic functions of eukaryotic cells1.

All of the genes of this simple eukaryotic cell have recently been revealed by an inter-national collaborative effort to determine the complete DNA sequence of its nuclear genome. Here we describe some of the fea-tures of. title = "Genome instability in rad54 mutants of Saccharomyces cerevisiae", abstract = "The RAD54 gene of Saccharomyces cerevisiae encodes a conserved dsDNA-dependent ATPase of the Swi2/Snf2 family with a specialized function during recombinational DNA by: 10nm of radiolabeled DNA substrate, M nucleotide(s) in 1× reaction buffer (2mM MgCl2, 50mM NaCl, 40mm Tris–HCl pH10mM dithiothreitol, g/ml bovine serum albumin, % glyc-erol).

All DNA substrates were confirmed to be >95% annealed by incubating. Sequence Details Sequence The S. cerevisiae Reference Genome sequence is derived from laboratory strain ad DNA or protein sequence, view genomic context and coordinates.

Click "Sequence Details" to view all sequence information for this locus, including that for other strains. Persistent Genomic Instability in the Yeast Saccharomyces cerevisiae Induced by Ionizing Radiation and DNA-Damaging Agents Richard J. Brennan and Robert H. Schiestl' Department of Cancer Cell Biology, Harvard School of Public Health, Huntington Avenue, Boston, Massachusetts Brennan, R.

and Schiestl, R. Persistent Genomic In. The Kluyveromyces linear plasmids, pGKL1 and pGKL2, carrying terminal protein (TP), are located in the cytoplasm and have a unique gene expression system with the plasmid-specific promoter element termed UCS, which functions only in the cytoplasm.

In this study we have developed an in vivo assay system in Saccharomyces cerevisiae which enables the detection of a rare migration Cited by: Systematic sequencing of the genome of Saccharomyces cerevisiae has revealed thousands of new predicted genes and allowed analysis of long-range features of chromosomal organization.

Generally, genes and predicted genes seem to be distributed evenly throughout the genome, having no overall preference for DNA strand. Summary.

The DNA of Saccharomyces exiguus was analyzed by Southern hybridization using cloned MATa, MATα, and HO genes of Saccharomyces cerevisiae as probes. It was shown that S. exiguus has a DNA sequence homologous with the HO gene of S.

cerevisiae and that this DNA sequence is on a chromosome of about kb of DNA in S. r, there is no DNA sequence in S. exiguus Cited by: 7. Nucleotide sequence asymmetry and codon adaptation index (CAI) for 15 SSD pairs in the S. cerevisiae sequence asymmetry measure on the x axis was calculated as the difference between unique nucleotide sites at the ancestral copy and the derived copy.

The y axis represents the difference in CAI values between the ancestral copy and the derived copy for the same Cited by: 4. A solo δ sequence flanking the 5′ end of the ADHII structural gene, ADR2, can promote a number of DNA rearrangements some of which were investigated in detail.

In a selective system haploid mutants were screened in which a solo S sequence flanking ADR2 had been joined to a Ty element. Three different types of events can create such a structure: Reintegration of a Ty sequence at the δ Cited by: 8.